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Telomeres, All You Need to Know in the Theory of Aging

October 25th, 2009

Telomeres are repetitive DNA sequences which protects the end of the human chromosome from destruction Molecular Biology of the Cell (wikipedia.org). Here, we provide you with information about telomeres in its ascociation with the theory of aging.

What you will find in this article are sections such DNA replication and telomere shortening as chromosom end replication problem and the consequences, the telomere’s functions, telomerase which stabilize telomere length, how does telomerase work, the telomere theory of aging (mechanistic explanation for the Hayflick limit), in vitro population doubling, the activity of telomerase, and Werner’s cellular phenotype reversed by telomerase.

Telomeres, All You Need to Know in the Theory of Aging continued »

H1N1 Article: How to Quantificate Swine Flu Human Pandemic Strain

October 23rd, 2009

The following H1N1 article is designed for general laboratory and research use only. It will give you informations Real-Time PCR Interpretation regarding in vitro quantification of “swine” H1N1 Influenza human pandemic strain through quantification kit for swine flu genomes.

In the depth of the article there are sections such What quantification kit used for, the kit contents, reagens and equipments, principle of the test (testing for influenza, Real-Time PCR, Positive and Negative Control, Internal RNA extraction control, Endogenous ACTB, Carry-over prevention using UNG), the Bench side Protocol, RNA extraction, Reverse Transcription protocol, Real-time PCR detection, and Amplification protocol.

H1N1 Article: How to Quantificate Swine Flu Human Pandemic Strain continued »

2008 Surgery Journal: Diagnostic and Management of Delirium in the ICU

October 21st, 2009

In the following article you will get information about the diagnostic criteria and scoring tools of delirium in the all about delirium intensive care unit (ICU). It gives you an overview of considerations for diagnosis and treatment of delirium, since patients often are misdiagnosed with anxiety and/or other disorder before making correct diagnosis for delirium.

Sections discussed in this journal are such the Clinical Features of Delirium, Three Dominant Types of Delirium (Agitated Delirium, Hypoactive Delirium, and Myxed Subtype), The CAM Diagnostic Algorithm, Nursing Delirium Screening Scale, The Delirium Detection Score (DDS), and The Therapeutic Considerations. All the sections focussed on the objective criteria and scoring system used to identify delirium in the ICU.

2008 Surgery Journal: Diagnostic and Management of Delirium in the ICU continued »

Rheumatology Article: Pathophysiology of Fibromyalgia

October 20th, 2009

This is a diagram of proposed interactions of various muscle abnormalities to pain and muscle dysfunction in fibromyalgia. It was hypothesized that any condition that could lead to constant muscle hypoxia and prolonged energy deficiency might be a cause of fibromyalgic pain.

Pathophysiology of Fibromyalgia Diagram

Rheumatology Article: Pathophysiology of Fibromyalgia continued »