Strong correlation of expression of surface antigens present when comparing myelogenous blasts from bone marrow and peripheral blood immunophenotypely. This medical journal describe whether this correlation also exists on the level of protein expression. 
The experts here investigated patients with newly diagnosed Acute Myeloid Leukimia (AML) using a proteomic approach with conventional two-dimensional (2D) electrophoresis and linear polyacrylamide gels.
AML is a severe hematological neoplasia that present by accumulating myeloid precursor cells in bone marrow and peripheral blood. A kind of proteomics emerges in AML diagnostic and therapy in the future, because its contribution in managements of AML is still negligible recently. Proteomics do contain 2D patterns and information from patients with lymphoproliverative disorders, leukemia, and other various cell populations.
The authors said that analysis of cell populations in vivo can result in protein changes that happen as a cause or consequences of the malignancy. Proteins hold as a key players in these cells more than other genes or mRNAs. Then the protein expression profiles of myelogenous blasts from AML’s patients were collected from bone marrow and peripheral blood. And the experts have observed that protein patterns from bone marrow and peripheral blood show a high correlation.
As those collected protein which came from different sites show basically not differ, it may indicate that samples of peripheral blood with high amounts of blasts are to be considered suitable for investigations of the proteome using 2D-electrophoresis. This is the conclusion of this medical journal which were written by Gero Hütter, Anne Letsch, Daniel Nowak, Julia Poland, Pranav Sinha, Eckhard Thiel, and Wolf-K Hofmann. Thanks to PubMed that provide us with Full Text Article and PDF Format.
