This article was issued by Standard Unit Department of Evaluations, Standard and Training Centre for Infections. It 
include standard operating procedures (SOPs), algorithms and guidance notes, promote high quality practices and help to assure the comparability of diagnostic information in different laboratories as National Standard Methods (NSM).
This article describes an algorithm refers to investigation where the case definition is met or where surveillance for H1N1 influenza A has been undertaken, the real time quadriplex PCR for the detection of influenza, negative PCR result consideration, One-step influenza multiple real time RT-PCR (for H1H3 subtype of influenza A), and also an algorithm description about the management of confirmed and probable cases (click to enlarge).
Download the National Standard Methods PCR for H1N1 Influenza A Investigation of pdf filetype, or you might see RT-PCR Specifity and Sensitivity in this blog (source: hpa-standardmethods.org.uk)
