Below are numbers of tests/tecniques help in the laboratory diagnostic of influenza. The techiques are classified as a laboratory diagnosis of the influenza virus direct and indirect diagnostic techniques. A Direct laboratory methods are direct immunofluorescence, ELISA, antigen capture and cells staining with monoclonal antibody that will detect viral particles, viral antigens, and viral genomes directly.

The indirect laboratory techniques are when the samples be inoculated in cell cultures, eggs, animals for growing the virus. But nowdays, molecular techniques are being popularly used for influenza virus diagnostics since it has sensitivity and quick turnround time such as RT-PCR, multiplex PCR, real time RT-PCR, NASBA (Nucleic Acid Based Amplification), and LAMP (Loop Mediated Isothermal Amplification).

We provide you in this following table various techniques used for the laboratory diagnosis of the influenza virus.

Procedure Flu Type Specimen Allowed Resulting Time
Viral Culture A and B Naso Pharingeal (NP)swab, throat swab, nasal wash, bronchial wash, nasal aspirate, sputum 3-10 days
Immuno-
fluorescence
A and B NP swab, nasal wash, bronchial wash, nasal aspirate, sputum 2-4 hours
RT-PCR A and B NP swab, throat swab, nasal wash, bronchial wash, nasal aspirate, sputum 2-4 hours
Serology A and B paired acute and convalescent serum samples 2 weeks or more
Enzyme Immuno Assay (EIA) Rapid Detection tests (using kits) A and B NP swab, throat swab, nasal wash, bronchial wash 2 hours
Directigen flu A A NP wash, and aspirate Less than 30 minutes